v0.4.0 introduced more robust task handling via the PRIMER_TASK flag, allowing the engine to act as a multi-purpose tool:
# Conda (Bioconda) conda install -c bioconda primer3=0.4.0 primer3 0.4.0
): Keep your pairs within a tight window (e.g., ). Aim for a maximum difference of 0.1°C between the forward and reverse primers to ensure they anneal simultaneously. primer3 0.4.0